Recombinant E.coli dsbE protein

DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Recombinant DsbE protein was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Thiol:disulfide interchange protein dsbE, CcmG, yejQ, Cytochrome c biogenesis protein ccmG, ECK2187, Periplasmic thioredoxin of cytochrome c type biogenesis, JW2183

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

18.1 kDa (161aa) confirmed by MALDI-TOF

Additionnal Information

DsbE, Thiol:disulfide interchange protein dsbE, CcmG, yejQ, Thiol:disulfide interchange protein dsbE Cytochrome c biogenesis protein ccmG..ECK2187, Periplasmic thioredoxin of cytochrome c type biogenesis, JW2183., ATGP0283-10 µg, ATGP0283-20 µg, ATGP0283-50 µg, ATGP0283-100 µg, ATGP0283-250 µg, ATGP0283-500 µg, ATGP0283-1 mg, ATGP0283-10, ATGP0283-20, ATGP0283-50, ATGP0283-100, ATGP0283-250, ATGP0283-500, ATGP0283-1

References & Citations

Reid E., et al. (2001) . Biochem J. 355 (1) :51-8.; ; Li Q., et al. (2001) . Biol Chem. 382 (12) :1679-86;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_416699

Uniprot Accession Number

P0AA86

Species

E.coli

AA Sequence

MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q