Recombinant human LMW-PTP/ACP1 protein

Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. Recombinant ACP1, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 15,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Product Name Alternative

Acid phosphatase 1 soluble isoform b, Acid phosphatase 1, soluble isoform b, ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatase, Acid phosphatase 1, soluble isoform b Acid phosphatase 1 soluble, Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase, Low molecular weight phosphotyrosine protein phosphatase, Acid phosphatase of erythrocyte, PAP2, Protein tyrosine phosphatase, PTPase, Purple acid phosphatase, Red cell acid phosphatase 1.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 15,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Molecular Weight

20.1 kDa (178aa) confirmed by MALDI-TOF

Additionnal Information

LMW-PTP, ACP1, Acid phosphatase 1 soluble isoform b, Acid phosphatase 1, soluble isoform b, ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatase, Acid phosphatase 1, soluble isoform b Acid phosphatase 1 soluble, Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase, Low molecular weight phosphotyrosine protein phosphatase, Acid phosphatase of erythrocyte, PAP2, Protein tyrosine phosphatase, PTPase, Purple acid phosphatase, Red cell acid phosphatase 1., ATGP0280-10 µg, ATGP0280-20 µg, ATGP0280-50 µg, ATGP0280-100 µg, ATGP0280-250 µg, ATGP0280-500 µg, ATGP0280-1 mg, ATGP0280-10, ATGP0280-20, ATGP0280-50, ATGP0280-100, ATGP0280-250, ATGP0280-500, ATGP0280-1

References & Citations

De Lorenzo A., et al. (2009) Metabolism.58 (3) :351-4; ; Banci M., et al. (2009) Cardiology.113 (4) :236-242.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 6.0) containing 0.1mM PMSF, 2mM EDTA, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_009030.1

Uniprot Accession Number

P24666

Species

Human

AA Sequence

MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH