Recombinant human DTNBP1 protein

Dysbindin is a ubiquitously expressed protein that binds to alpha- and beta-dystrobrevins, components of the dystrophin-associated protein complex (DPC) in both muscle and nonmuscle cells. Dysbindin has been strongly implicated in schizophrenia (SZ) susceptibility by a series of independent genetic association and gene expression studies. Among its known functions, dysbindin is part of a protein complex, termed the biogenesis of lysosome-related organelles complex 1 (BLOC-1), the molecular components of which might be involved in the regulation of vesicular trafficking and dendrite branching. Dysbindin effects for brain volume and cortical thickness appear driven by different neurobiological processes. Recombinant human Dysbindin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Dystrobrevin-binding protein 1 isoform C, DTNBP1, DBND, HPS7, SDY, Dystrobrevin-binding protein 1 isoform C Dystrobrevin binding protein 1, Hermansky Pudlak syndrome 7 protein, Dysbindin

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.5 kDa (306aa) confirmed by MALDI-TOF

Additionnal Information

Dysbindin, Dystrobrevin-binding protein 1 isoform C, DTNBP1, DBND, HPS7, SDY, Dystrobrevin-binding protein 1 isoform C Dystrobrevin binding protein 1, Hermansky Pudlak syndrome 7 protein., ATGP0278-10 µg, ATGP0278-20 µg, ATGP0278-50 µg, ATGP0278-100 µg, ATGP0278-250 µg, ATGP0278-500 µg, ATGP0278-1 mg, ATGP0278-10, ATGP0278-20, ATGP0278-50, ATGP0278-100, ATGP0278-250, ATGP0278-500, ATGP0278-1

References & Citations

Li W., et al. (2003) . Nat Genet. 35 (1) :84-9; ; Chen XW., et al. (2008) . J Cell Biol. 181 (5) :791-801;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 0.5mM DTT, 20% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_898862.1

Uniprot Accession Number

Q96EV8

Species

Human

AA Sequence

MLSA HWEKKKTSLV ELQEQLQQLP ALIADLESMT ANLTHLEASF EEVENNLLHL EDLCGQCELE RCKHMQSQQ LENYKKNKRKE LETFKAELDA EHAQKVLEME HTQQMKLKER QKFFEEAFQQ DMEQYLSTGY LQIAERREPI GSMSSMEVNV DMLEQMDLMD ISDQEALDVF LNSGGEENTV LSPALGPESS TCQNEITLQV PNPSELRAKP PSSSSTCTDS ATRDISEGGE SPVVQSDEEE VQVDTALATS HTDREATPDG GEDSDS