Recombinant human GRP94/HSP90B1 protein

GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. Recombinant human GRP94 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

94.4 kDa (819aa)

Additionnal Information

Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GRP9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein, 94 kDa glucose regulated protein, 94kDa glucose regulated protein, 94kDa glucose-regulated protein, ATGP0276-10 µg, ATGP0276-20 µg, ATGP0276-50 µg, ATGP0276-100 µg, ATGP0276-250 µg, ATGP0276-500 µg, ATGP0276-1 mg, ATGP0276-010, ATGP0276-020, ATGP0276-050, ATGP0276-100, ATGP0276-250, ATGP0276-500, ATGP0276-01M

References & Citations

Randow F., et al. (2001) . Nat Cell Biol. 3 (10) :E231.; ; Li Z., et al. (2002) . Front Biosci. 7:d731-51. ;

Other References

Yoshimura C, et al. Thermodynamic Dissection of Potency and Selectivity of Cytosolic Hsp90 Inhibitors. (J Med Chem. 2021) {https://pubmed.ncbi.nlm.nih.gov/33621080/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 1mM EDTA, 0.1M NaCl, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_003290.1

Uniprot Accession Number

P14625

Species

Human

AA Sequence

DDE VDVDGTVEED LGKSREGSRT DDEVVQREEE AIQLDGLNAS QIRELREKSE KFAFQAEVNR MMKLIINSLY KNKEIFLREL ISNASDALDK IRLISLTDEN ALSGNEELTV KIKCDKEKNL LHVTDTGVGM TREELVKNLG TIAKSGTSEF LNKMTEAQED GQSTSELIGQ FGVGFYSAFL VADKVIVTSK HNNDTQHIWE SDSNEFSVIA DPRGNTLGRG TTITLVLKEE ASDYLELDTI KNLVKKYSQF INFPIYVWSS KTETVEEPME EEEAAKEEKE ESDDEAAVEE EEEEKKPKTK KVEKTVWDWE LMNDIKPIWQ RPSKEVEEDE YKAFYKSFSK ESDDPMAYIH FTAEGEVTFK SILFVPTSAP RGLFDEYGSK KSDYIKLYVR RVFITDDFHD MMPKYLNFVK GVVDSDDLPL NVSRETLQQH KLLKVIRKKL VRKTLDMIKK IADDKYNDTF WKEFGTNIKL GVIEDHSNRT RLAKLLRFQS SHHPTDITSL DQYVERMKEK QDKIYFMAGS SRKEAESSPF VERLLKKGYE VIYLTEPVDE YCIQALPEFD GKRFQNVAKE GVKFDESEKT KESREAVEKE FEPLLNWMKD KALKDKIEKA VVSQRLTESP CALVASQYGW SGNMERIMKA QAYQTGKDIS TNYYASQKKT FEINPRHPLI RDMLRRIKED EDDKTVLDLA VVLFETATLR SGYLLPDTKA YGDRIERMLR LSLNIDPDAK VEEEPEEEPE ETAEDTTEDT EQDEDEEMDV GTDEEEETAK ESTAEKDEL