Recombinant human eIF-5A1/EIF5A protein

Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer. Recombinant human eIF5A protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 5A-1 isoform B, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.8 kDa (154aa) confirmed by MALDI-TOF

Additionnal Information

EIF5A, Eukaryotic translation initiation factor 5A isoform B, EIF-5A, EIF5A1, eIF5AI, Eukaryotic translation initiation factor 5A isoform B eIF 4D, EIF 5A, eIF 5A 1, eIF 5A1, eIF4D, uORF, uORF A, Eukaryotic initiation factor 5A, Rev binding factor, Eukaryotic initiation factor 5A isoform 1, MGC99547, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A 1, MGC104255., ATGP0272-10 µg, ATGP0272-20 µg, ATGP0272-50 µg, ATGP0272-100 µg, ATGP0272-250 µg, ATGP0272-500 µg, ATGP0272-1 mg, ATGP0272-10, ATGP0272-20, ATGP0272-50, ATGP0272-100, ATGP0272-250, ATGP0272-500, ATGP0272-1

References & Citations

Saini P, et al. (2009) . Nature. 459 (7243) :118-21; ; Gosslau A., et al. (2009) . J Cell Physiol. 219 (2) :485-93;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM Tris-HCl buffer (pH 7.5) containing 10 % glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_001961

Uniprot Accession Number

P63241

Species

Human

AA Sequence

MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK