Recombinant mouse IL-18 protein

Interleukin 18 (IL-18), previously known as interferon-gamma (IFN-gamma) -inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

Interleukin 18, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Igif

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.4 kDa (178aa) confirmed by MALDI-TOF

Additionnal Information

IL18, IL1F4, Interleukin-1 gamma, IL-1 gamma, ATGP0271-10 µg, ATGP0271-20 µg, ATGP0271-50 µg, ATGP0271-100 µg, ATGP0271-250 µg, ATGP0271-500 µg, ATGP0271-1 mg, ATGP0271-010, ATGP0271-020, ATGP0271-050, ATGP0271-100, ATGP0271-250, ATGP0271-500, ATGP0271-01M

References & Citations

Okamura H., et al. (1995) Nature, 378, 88-91. ; ; Yoshimoto T., et al. (1998) J. Immunol, 161, 3400. ; ; Gracie JA ., et al. (2003) J Leukoc Biol. 73,213.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_032386

Uniprot Accession Number

P70380

Species

Mouse

AA Sequence

MNFGRLHCTT AVIRNINDQV LFVDKRQPVF EDMTDIDQSA SEPQTRLIIY MYKDSEVRGL AVTLSVKDSK MSTLSCKNKI ISFEEMDPPE NIDDIQSDLI FFQKRVPGHN KMEFESSLYE GHFLACQKED DAFKLILKKK DENGDKSVMF TLTNLHQS