Recombinant human DKK1 protein

Dickkopf-related protein 1, also known as Dkk-1, is a member of the Dkk protein family including Dkk-1, 2, 3 and -4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated level of Dkk-1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Recombinant human Dkk-1 protein, fused to His-tag at C-terminus, was expressed ininsect cell using baculovirus expression system and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Dickkopf WNT signaling pathway inhibitor 1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, SK

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

27.8 kDa (253aa)

Additionnal Information

Dickkopf 1, Dickkopf 1 homolog, Dickkopf 1 like, Dickkopf homolog 1, Dickkopf related protein 1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf-related protein 1 Dickkopf 1, DKK 1, DKK1, DKK-1, hDkk 1, hDkk1, hDkk-1, SK, Xenopus laevis, ATGP0269-10 µg, ATGP0269-20 µg, ATGP0269-50 µg, ATGP0269-100 µg, ATGP0269-250 µg, ATGP0269-500 µg, ATGP0269-1 mg, ATGP0269-010, ATGP0269-020, ATGP0269-050, ATGP0269-100, ATGP0269-250, ATGP0269-500, ATGP0269-01M

References & Citations

Krupnik, V.E. et al. (1999) Gene 238:301-13. ; ; Nusse, R. et al. (2001) Nature 411:255-6. ; ; Mao, J. et al. (2001) Mol. Cell 7:801-9.;

Other References

Hino M, et al. Transforming growth factor-β1 induces invasion ability of HSC-4 human oral squamous cell carcinoma cells through the Slug/Wnt-5b/MMP-10 signalling axis. (J Biochem. 2016) {https://pubmed.ncbi.nlm.nih.gov/26861993/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol, 2mM DTT, 2mM EDTA, 0.1mM PMSF

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036374

Uniprot Accession Number

O94907

Species

Human

N Terminal Sequence

Ala

AA Sequence

TLNSVLN SNAIKNLPPP LGGAAGHPGS AVSAAPGILY PGGNKYQTID NYQPYPCAED EECGTDEYCA SPTRGGDAGV QICLACRKRR KRCMRHAMCC PGNYCKNGIC VSSDQNHFRG EIEETITESF GNDHSTLDGY SRRTTLSSKM YHTKGQEGSV CLRSSDCASG LCCARHFWSK ICKPVLKEGQ VCTKHRRKGS HGLEIFQRCY CGEGLSCRIQ KDHHQASNSS RLHTCQRHSG RLVPRGS