Recombinant human DKK1 protein
Dickkopf-related protein 1, also known as Dkk-1, is a member of the Dkk protein family including Dkk-1, 2, 3 and -4. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated level of Dkk-1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Recombinant human Dkk-1 protein, fused to His-tag at C-terminus, was expressed ininsect cell using baculovirus expression system and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Dickkopf WNT signaling pathway inhibitor 1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, SK
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Molecular Weight
27.8 kDa (253aa)
Additionnal Information
References & Citations
Krupnik, V.E. et al. (1999) Gene 238:301-13. ; ; Nusse, R. et al. (2001) Nature 411:255-6. ; ; Mao, J. et al. (2001) Mol. Cell 7:801-9.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol, 2mM DTT, 2mM EDTA, 0.1mM PMSF
Scientific Category
Signal Transduction
NCBI Accession Number
NP_036374
Uniprot Accession Number
O94907
Species
Human
N Terminal Sequence
Ala
AA Sequence
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