Recombinant human Dopa Decarboxylase/DDC protein

Dopa decarboxylase, also known as DDC, is a homodimeric, pyridoxal phosphate dependent enzyme. Dopa decarboxylase is a protein implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Dopa decarboxylase is found in different areas of the brain and is particularly abundant in basal ganglia. Recombinant human Dopa decarboxylase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

DDC, AADC, Aromatic L-amino acid decarboxylase, L-Dopa decarboxylase, Aromatic L Amino Acid Decarboxylase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

56.4 kDa (503aa) confirmed by MALDI-TOF

Additionnal Information

Dopa Decarboxylase, DDC, DDC, AADC, Aromatic L-amino acid decarboxylase, L-Dopa decarboxylase, Aromatic L Amino Acid Decarboxylase, ATGP0267-10 µg, ATGP0267-20 µg, ATGP0267-50 µg, ATGP0267-100 µg, ATGP0267-250 µg, ATGP0267-500 µg, ATGP0267-1 mg, ATGP0267-10, ATGP0267-20, ATGP0267-50, ATGP0267-100, ATGP0267-250, ATGP0267-500, ATGP0267-1

References & Citations

Avgeris M., et al. (2008) . Clin Biochem. 41 (14-15) :1140-9.; ; Pons R., et al. (2004) . Neruology. 62 (7) :1058-65.;

Other References

Uenishi E, et al. A novel mechanism of idiopathic orthostatic hypotension and hypocatecholaminemia due to autoimmunity against aromatic l-Amino acid decarboxylase. (Biochem Biophys Res Commun. 2024) {https://pubmed.ncbi.nlm.nih.gov/38677008/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_001076440.2

Uniprot Accession Number

P20711

Species

Human

AA Sequence

MNASEFR RRGKEMVDYV ANYMEGIEGR QVYPDVEPGY LRPLIPAAAP QEPDTFEDII NDVEKIIMPG VTHWHSPYFF AYFPTASSYP AMLADMLCGA IGCIGFSWAA SPACTELETV MMDWLGKMLE LPKAFLNEKA GEGGGVIQGS ASEATLVALL AARTKVIHRL QAASPELTQA AIMEKLVAYS SDQAHSSVER AGLIGGVKLK AIPSDGNFAM RASALQEALE RDKAAGLIPF FMVATLGTTT CCSFDNLLEV GPICNKEDIW LHVDAAYAGS AFICPEFRHL LNGVEFADSF NFNPHKWLLV NFDCSAMWVK KRTDLTGAFR LDPTYLKHSH QDSGLITDYR HWQIPLGRRF RSLKMWFVFR MYGVKGLQAY IRKHVQLSHE FESLVRQDPR FEICVEVILG LVCFRLKGSN KVNEALLQRI NSAKKIHLVP CHLRDKFVLR FAICSRTVES AHVQRAWEHI KELAADVLRA ERE