Raw264.7 Lysate
Raw264. 7 cells are established from ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV) . This line does not secrete detectable virus particles and is negative in the XC plaque formation assay. Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.
Product Specifications
Antigen Species
Mouse
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Mouse
WB Description
All lanes : Raw264.7 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody
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