WiDr Lysate
WiDr cells are established from a 78 year old female with colon adenocarcinoma. The cells are negative for colon antigen 3 expression and positive for keratin by immunoperoxidase staining. WiDr cells expressed p53 antigen. Growth of WiDR cells is inhibited by tumor necrosis factor alpha (TNF-alpha) . Inhibitors of dihydrofolate reductase are highly cytotoxic to WiDR cells.
Product Specifications
Antigen Species
Human
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Human
WB Description
All lanes : WiDr Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody
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