A431 Lysate
A431 cells were established from an epidermoid carcinoma in the skin/epidermis of an 85 year old female patient. A431 cells are used in studies of the cell cycle and cancer-associated cell signalling pathways since they express abnormally high levels of the EGFR. As such they are often used as a positive control for EGFR expression.
Product Specifications
Antigen Species
Human
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Human
WB Description
All lanes : A431 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody
Explore Other Products
Browse additional items from our catalog
Rabbit Polyclonal AlphaB Crystallin/CRYAB Antibody [Alexa Fluor 594]
