MIS12 cDNA

MIS12 is a component of the MIS12 complex, which is required for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex consists of MIS12, DSN1, NSL1 and PMF-1. MIS12 is part of a network of complexes that provide microtubule attachment and generates pulling forces from depolymerization.

Product Specifications

Product Name Alternative

2510025F08Rik, hMis12, KNTC2AP, MTW1

Chromosomal Location

17p13.2

Antigen Species

Human

Vector Type

PATGen (puc19-derived cloning vector)

Sequence

ATGTCTGTGGATCCAATGACCTACGAGGCCCAGTTCTTTGGCTTCACGCCACAAACGTGCATGCTTCGGATCTACATTGCATTTCAAGACTACCTATTTGAAGTGATGCAGGCCGTTGAACAGGTTATTCTGAAGAAGCTGGATGGCATCCCAGACTGTGACATTAGCCCAGTGCAGATTCGCAAATGCACAGAGAAGTTTCTTTGCTTCATGAAAGGACATTTTGATAACCTTTTTAGCAAAATGGAGCAACTGTTTTTGCAGCTGATTTTACGTATTCCCTCAAACATCTTGCTTCCTGAAGATAAATGTAAGGAGACACCTTATAGTGAGGAAGATTTTCAGCATCTCCAGAAAGAAATTGAACAGTTACAGGAGAAGTACAAGACTGAATTATGTACTAAGCAGGCCCTTCTTGCAGAATTAGAAGAGCAAAAAATTGTTCAGGCCAAACTCAAACAGACGTTGACTTTCTTTGATGAGCTTCATAATGTTGGCAGAGATCATGGGACTAGTGATTTTAGGGAGAGTTTAGTATCCCTGGTTCAGAACTCCAGAAAACTACAGAACATTAGAGACAATGTGGAAAAGGAATCGAAACGACTGAAAATATCTTAA

Additionnal Information

MIS12, 2510025F08Rik, hMis12, KNTC2AP, MTW1, MIS12, ATGD0332-10 µg, ATGD0332-20 µg, ATGD0332-50 µg, ATGD0332-100 µg, ATGD0332-250 µg, ATGD0332-500 µg, ATGD0332-1 mg, ATGD0332-10, ATGD0332-20, ATGD0332-50, ATGD0332-100, ATGD0332-250, ATGD0332-500, ATGD0332-1

Storage Conditions

Store the plasmid at -20C.

Formulation

Lyophilized

Scientific Category

Cell Cycle

Omim

609178

NCBI Accession Number

NP_076944.1

Species

Human

Preparation

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

RNA Reference

NM_024039.2

DNA Size

618bp

Vector Description

This shuttle vector contains the complete ORF. It is inseted Nde I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.