ARL3 cDNA

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP) . Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit.

Product Specifications

Product Name Alternative

ARFL3

Chromosomal Location

10q23.3

Antigen Species

Human

Vector Type

PATGen (puc19-derived cloning vector)

Sequence

ATGGGCTTGCTCTCAATTTTGCGCAAGTTGAAAAGTGCACCAGACCAGGAGGTGAGAATACTTCTCCTGGGCTTGGATAATGCTGGCAAGACCACTCTTCTGAAGCAGCTTGCATCTGAAGACATCAGCCACATCACACCTACACAGGGTTTCAACATCAAAAGTGTACAATCACAAGGTTTTAAACTGAATGTATGGGACATTGGTGGACAGAGGAAAATCAGACCATACTGGAAGAATTATTTTGAAAATACCGATATTCTTATATATGTAATCGACAGTGCAGACAGAAAAAGATTTGAAGAGACGGGTCAGGAACTAGCGGAATTACTGGAGGAAGAAAAACTAAGTTGTGTGCCAGTGCTCATCTTTGCTAATAAGCAGGATTTGCTCACAGCAGCCCCTGCCTCTGAAATTGCAGAAGGACTGAACCTGCATACCATCCGCGACCGAGTCTGGCAGATCCAGTCTTGCTCAGCTCTCACAGGAGAGGGCGTTCAGGATGGCATGAACTGGGTCTGCAAAAATGTCAATGCAAAGAAGAAATAA

Additionnal Information

ARL3, ARFL3, ARL3, ATGD0196-10 µg, ATGD0196-20 µg, ATGD0196-50 µg, ATGD0196-100 µg, ATGD0196-250 µg, ATGD0196-500 µg, ATGD0196-1 mg, ATGD0196-10, ATGD0196-20, ATGD0196-50, ATGD0196-100, ATGD0196-250, ATGD0196-500, ATGD0196-1

Storage Conditions

Store the plasmid at -20C.

Formulation

Lyophilized

Scientific Category

Cancer

Omim

604695

NCBI Accession Number

NP_004302.1

Species

Human

Preparation

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

RNA Reference

NM_004311.3

DNA Size

549bp

Vector Description

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.