DPM1 cDNA

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER) . Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2

Product Specifications

Product Name Alternative

CDGIE, MPDS

Chromosomal Location

20q13.13

Antigen Species

Human

Vector Type

PATGen (puc19-derived cloning vector)

Sequence

ATGGCCTCCT TGGAAGTCAG TCGTAGTCCT CGCAGGTCTC GGCGGGAGCT GGAAGTGCGC AGTCCACGAC AGAACAAATA TTCGGTGCTT TTACCTACCT ACAACGAGCG CGAGAACCTG CCGCTCATCG TGTGGCTGCT GGTGAAAAGC TTCTCCGAGA GTGGAATCAA CTATGAAATT ATAATCATAG ATGATGGAAG CCCAGATGGA ACAAGGGATG TTGCTGAACA GTTGGAGAAG ATCTATGGGT CAGACAGAAT TCTTCTAAGA CCACGAGAGA AAAAGTTGGG ACTAGGAACT GCATATATTC ATGGAATGAA ACATGCCACA GGAAACTACA TCATTATTAT GGATGCTGAT CTCTCACACC ATCCAAAATT TATTCCTGAA TTTATTAGGA AGCAAAAGGA GGGTAATTTT GATATTGTCT CTGGAACTCG CTACAAAGGA AATGGAGGTG TATATGGCTG GGATTTGAAA AGAAAAATAA TCAGCCGTGG GGCCAATTTT TTAACTCAGA TCTTGCTGAG ACCAGGAGCA TCTGATTTAA CAGGAAGTTT CAGATTATAC CGAAAAGAAG TTCTAGAGAA ATTAATAGAA AAATGTGTTT CTAAAGGCTA CGTCTTCCAG ATGGAGATGA TTGTTCGGGC AAGACAGTTG AATTATACTA TTGGCGAGGT TCCAATATCA TTTGTGGATC GTGTTTATGG TGAATCCAAG TTGGGAGGAA ATGAAATAGT ATCTTTCTTG AAAGGATTAT TGACTCTTTT TGCTACTACA TAA

Additionnal Information

DPM1, CDGIE, MPDS, DPM1, ATGD0064-10 µg, ATGD0064-20 µg, ATGD0064-50 µg, ATGD0064-100 µg, ATGD0064-250 µg, ATGD0064-500 µg, ATGD0064-1 mg, ATGD0064-10, ATGD0064-20, ATGD0064-50, ATGD0064-100, ATGD0064-250, ATGD0064-500, ATGD0064-1

Storage Conditions

Store the plasmid at -20C.

Formulation

Lyophilized

Scientific Category

Tags & Cell Markers

Omim

603503

NCBI Accession Number

NP_003850.1

Species

Human

Preparation

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

RNA Reference

NM_003859.1

DNA Size

783bp

Vector Description

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.