BCAS2 cDNA

BCAS2, also known as pre-mRNA-splicing factor SPF27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. It is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TRbeta (thyroid hormone receptor beta), ERalpha (estrogen receptor alpha), ERbeta, PR (progesterone receptor) and PPARgamma (peroxisome proliferator-activated receptor gamma) . This protein functions as an ER co-activator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer.

Product Specifications

Product Name Alternative

DAM1, Snt309, SPF27

Chromosomal Location

1q13.2

Antigen Species

Human

Vector Type

PATGen (puc19-derived cloning vector)

Sequence

ATGGCGGGCA CAGGTTTGGT GGCTGGAGAG GTTGTGGTGG ATGCGCTGCC GTATTTTGAT CAAGGTTATG AAGCCCCTGG TGTGCGGGAA GCGGCTGCAG CGCTGGTGGA GGAGGAAACT CGCAGATACC GACCTACTAA GAACTACCTG AGCTACCTGA CAGCCCCGGA TTATTCTGCC TTTGAAACTG ACATAATGAG AAATGAATTT GAAAGACTGG CTGCTCGACA ACCAATTGAA TTGCTCAGTA TGAAACGATA TGAGCTTCCA GCCCCCTCCT CTGGTCAAAA AAATGACATT ACTGCATGGC AAGAATGTGT AAACAATTCT ATGGCCCAGT TAGAGCATCA AGCAGTTAGA ATTGAGAATC TGGAACTAAT GTCACAGCAT GGATGTAATG CCTGGAAAGT ATACAATGAA AATCTAGTTC ATATGATTGA ACACGCACAG AAGGAACTTC AGAAGTTAAG AAAACATATT CAAGATTTAA ACTGGCAGAG AAAGAACATG CAACTCACAG CTGGATCTAA ATTGAGAGAA ATGGAGTCAA ATTGGGTATC CCTGGTCAGT AAGAATTATG AGATTGAACG GACTATTGTT CAGCTAGAAA ATGAAATCTA TCAAATTAAG CAGCAACATG GAGAGGCAAA CAAAGAAAAC ATCCGGCAAG ACTTCTGA

Additionnal Information

BCAS2, DAM1, Snt309, SPF27, BCAS2, ATGD0062-10 µg, ATGD0062-20 µg, ATGD0062-50 µg, ATGD0062-100 µg, ATGD0062-250 µg, ATGD0062-500 µg, ATGD0062-1 mg, ATGD0062-10, ATGD0062-20, ATGD0062-50, ATGD0062-100, ATGD0062-250, ATGD0062-500, ATGD0062-1

Storage Conditions

Store the plasmid at -20C.

Formulation

Lyophilized

Scientific Category

Cancer

Omim

605783

NCBI Accession Number

NP_005863.1

Species

Human

Preparation

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

RNA Reference

NM_005872.2

DNA Size

678bp

Vector Description

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.