HCK cDNA

HCK encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon.

Product Specifications

Product Name Alternative

Tyrosine-protein kinase HCK, HCK proto-oncogene, Src family tyrosine kinase, Hemopoietic cell kinase, JTK9, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck

Chromosomal Location

20q11-q12

Antigen Species

Human

Vector Type

PATGen (puc19-derived cloning vector)

Sequence

CTGGGGGGGC GCTCAAGCTG CGAGGATCCG GGCTGCCCGC GAGACGAGGA GCGGGCGCCC AGGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGCAGG CTCTGAGGAC ATCATCGTGG TTGCCCTGTA TGATTACGAG GCCATTCACC ACGAAGACCT CAGCTTCCAG AAGGGGGACC AGATGGTGGT CCTAGAGGAA TCCGGGGAGT GGTGGAAGGC TCGATCCCTG GCCACCCGGA AGGAGGGCTA CATCCCAAGC AACTATGTCG CCCGCGTTGA CTCTCTGGAG ACAGAGGAGT GGTTTTTCAA GGGCATCAGC CGGAAGGACG CAGAGCGCCA ACTGCTGGCT CCCGGCAACA TGCTGGGCTC CTTCATGATC CGGGATAGCG AGACCACTAA AGGAAGCTAC TCTTTGTCCG TGCGAGACTA CGACCCTCGG CAGGGAGATA CCGTGAAACA TTACAAGATC CGGACCCTGG ACAACGGGGG CTTCTACATA TCCCCCCGAA GCACCTTCAG CACTCTGCAG GAGCTGGTGG ACCACTACAA GAAGGGGAAC GACGGGCTCT GCCAGAAACT GTCGGTGCCC TGCATGTCTT CCAAGCCCCA GAAGCCTTGG GAGAAAGATG CCTGGGAGAT CCCTCGGGAA TCCCTCAAGC TGGAGAAGAA ACTTGGAGCT GGGCAGTTTG GGGAAGTCTG GATGGCCACC TACAACAAGC ACACCAAGGT GGCAGTGAAG ACGATGAAGC CAGGGAGCAT GTCGGTGGAG GCCTTCCTGG CAGAGGCCAA CGTGATGAAA ACTCTGCAGC ATGACAAGCT GGTCAAACTT CATGCGGTGG TCACCAAGGA GCCCATCTAC ATCATCACGG AGTTCATGGC CAAAGGAAGC TTGCTGGACT TTCTGAAAAG TGATGAGGGC AGCAAGCAGC CATTGCCAAA ACTCATTGAC TTCTCAGCCC AGATTGCAGA AGGCATGGCC TTCATCGAGC AGAGGAACTA CATCCACCGA GACCTCCGAG CTGCCAACAT CTTGGTCTCT GCATCCCTGG TGTGTAAGAT TGCTGACTTT GGCCTGGCCC GGGTCATTGA GGACAACGAG TACACGGCTC GGGAAGGGGC CAAGTTCCCC ATCAAGTGGA CAGCTCCTGA AGCCATCAAC TTTGGCTCCT TCACCATCAA GTCAGACGTC TGGTCCTTTG GTATCCTGCT GATGGAGATC GTCACCTACG GCCGGATCCC TTACCCAGGG ATGTCAAACC CTGAAGTGAT CCGAGCTCTG GAGCGTGGAT ACCGGATGCC TCGCCCAGAG AACTGCCCAG AGGAGCTCTA CAACATCATG ATGCGCTGCT GGAAAAACCG TCCGGAGGAG CGGCCGACCT TCGAATACAT CCAGAGTGTG CTGGATGACT TCTACACGGC CACAGAGAGC CAGTACCAAC AGCAGCCATG A

Additionnal Information

HCK, HCK proto oncogene, HCK proto-oncogene, Hemopoietic cell kinase, JTK9, P08631, p59 HCK, p59 HCK/p60 HCK, p59HCk, p59-HCK/p60-HCK, p60 HCK, p60HCK, p61 HCK, p61HCK, Src family tyrosine kinase, Tyrosine protein kinase HCK, Tyrosine-protein kinase HCK, ATGD0014-10 µg, ATGD0014-20 µg, ATGD0014-50 µg, ATGD0014-100 µg, ATGD0014-250 µg, ATGD0014-500 µg, ATGD0014-1 mg, ATGD0014-010, ATGD0014-020, ATGD0014-050, ATGD0014-100, ATGD0014-250, ATGD0014-500, ATGD0014-01M

Storage Conditions

Store the plasmid at -20C.

Formulation

Lyophilized

Scientific Category

Immunology

Omim

142370

NCBI Accession Number

NP_002101.2

Species

Human

Preparation

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

RNA Reference

NM_002110.3

DNA Size

1581bp

Vector Description

This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.