Human beta-Actin antibody

Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

Product Specifications

Product Name Alternative

Actin, cytoplasmic 1, Beta-actin

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACTB (1-375aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4G6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

0.2 mg/mL (determined by BCA assay)

Additionnal Information

B-Actin, beta actin, beta-actin, actin, ATGA0570-10 µg, ATGA0570-20 µg, ATGA0570-50 µg, ATGA0570-100 µg, ATGA0570-250 µg, ATGA0570-500 µg, ATGA0570-1 mg, ATGA0570-010, ATGA0570-020, ATGA0570-050, ATGA0570-100, ATGA0570-250, ATGA0570-500, ATGA0570-01M, ATGA0606-10 µg, ATGA0606-20 µg, ATGA0606-50 µg, ATGA0606-100 µg, ATGA0606-250 µg, ATGA0606-500 µg, ATGA0606-1 mg, ATGA0606-010, ATGA0606-020, ATGA0606-050, ATGA0606-100, ATGA0606-250, ATGA0606-500, ATGA0606-01M

References & Citations

Gunning PW., et al. (2015) Journal of Cell Science. 128 (11) : 2009-2019.; ; Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163 (4) : 673-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_001092

Uniprot Accession Number

P60709

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: PC3 cell lysate; ; Lane 4.: HepG2 cell lysate; ; Lane 5.: MCF7 cell lysate; ; Lane 6.: U87-MG cell lysate; ; Lane 7.: NIH-3T3 cell lysateThe mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Kidney tissue lysate; ; Lane 2.: Spleen tissue lysate; ; Lane 3.: Bone marrow tissue lysate; ; Lane 4.: Stomach tissue lysate; ; Lane 5.: Liver tissue lysate {2503310001584356.jpg}The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: ACTB antibody 1:1000; ; Lane 2.: ACTB antibody 1:3000; ; Lane 3.: ACTB antibody 1:5000; ; Lane 4.: ACTB antibody 1:10000 {2503310001814296.jpg}

IF Description

ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .