Human AGXT antibody

The AGXT gene provides instructions for making a liver enzyme called alanine-glyoxylate aminotransferase. Inside liver cells, this enzyme is found in peroxisomes, structures that contain many different enzymes used to produce energy and the basic materials important for cellular activities. AGXT converts a compound called glyoxylate to glycine, an amino acid that is a building block for making enzymes and other proteins.

Product Specifications

Product Name Alternative

Alanine-glyoxylate aminotransferase, AGXT1, PH1, AGT, SPT, AGT1, L-alanine: glyoxylate aminotransferase 1, serine:pyruvate aminotransferase, Ser-PyrAT, SPAT, TLH6

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant AGXT (330-392aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2D10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL

Additionnal Information

ATGA0604-10 µg, ATGA0604-20 µg, ATGA0604-50 µg, ATGA0604-100 µg, ATGA0604-250 µg, ATGA0604-500 µg, ATGA0604-1 mg, ATGA0604-010, ATGA0604-020, ATGA0604-050, ATGA0604-100, ATGA0604-250, ATGA0604-500, ATGA0604-01M, ATGA0166-10 µg, ATGA0166-20 µg, ATGA0166-50 µg, ATGA0166-100 µg, ATGA0166-250 µg, ATGA0166-500 µg, ATGA0166-1 mg, ATGA0166-010, ATGA0166-020, ATGA0166-050, ATGA0166-100, ATGA0166-250, ATGA0166-500, ATGA0166-01M

References & Citations

Donini S, et al., (2009) Biochem J 422 (2) :265-272.; ; Grujic D, et al., (2009) Am J Nephrol 29 (2) :86-93.; ; Williams EL, et al., (2009) Hum Mutat 30 (6) :910-917.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_000021.1

Uniprot Accession Number

P21549

WB Description

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AGXT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human AGXT protein

FACS Description

Flow cytometry analysis of AGXT in HeLa cells. The cell was stained with ATGA0604 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of AGXT in HeLa cells. The cell was stained with ATGA0604 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of AGXT in Hep3B cells. The cell was stained with ATGA0604 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2404230001993811.jpg}