Human RAC 1/2/3 antibody

Rac is a subfamily of the Rho family of GTPases, small (~21 kDa) signaling G proteins (more specifically a GTPase) . When bound to GTP, Rac is activated. In its activated state, Rac participates in the regulation of cell movement, through its involvement in structural changes to the actin Cytoskeleton. Rac is small GTPases that have been linked to multiple human cancers and are implicated in epithelial to mesenchymal transition, cell-cycle progression, migration/invasion, tumor growth, angiogenesis, and oncogenic transformation.

Product Specifications

Product Name Alternative

As-related C3 botulinum toxin substrate 3, Cell migration-inducing gene 5 protein, EN-7, Gx, HSPC022, MIG 5, MIG5, Migration inducing gene 5, Migration inducing protein 5, p21 Rac1, p21 Rac2, p21 Rac3, RAC1, Rac1B, RAC2, RAC3, Ras like protein TC25, Ras related C3 botulinum substrate 1, Ras related C3 botulinum substrate 2, Ras related C3 botulinum substrate 3, Ras related C3 botulinum toxin substrate 1, Ras related C3 botulinum toxin substrate 1 isoform Rac1, Ras related C3 botulinum toxin substrate 1 isoform Rac1 MGC111543, Ras related C3 botulinum toxin substrate 2, Ras related C3 botulinum toxin substrate 3, Rho family small GTP binding protein Rac1, Rho family small GTP binding protein Rac2, Rho family small GTP binding protein Rac3, RP23-84C12.18, Small G protein, TC 25

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human RAC2 (1-189aa) protein purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2G10

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

As-related C3 botulinum toxin substrate 3, Cell migration-inducing gene 5 protein, EN 7, EN7, EN-7, Gx, HSPC 022, HSPC022, MIG 5, MIG5, Migration inducing gene 5, Migration inducing protein 5, p21 Rac 2, p21 Rac1, p21 Rac2, p21-Rac1, p21Rac2, p21-Rac3, Rac 1, RAC 2, RAC1, Rac1B, Ras like protein TC25, RAS related C3 botulinum substrate 3, Ras related C3 botulinum toxin substrate 1, Ras related C3 botulinum toxin substrate 2, Ras related C3 botulinum toxin substrate 3, Ras-like protein TC25, Ras-related C3 botulinum toxin substrate 1, Ras-related C3 botulinum toxin substrate 1 isoform Rac1, Ras-related C3 botulinum toxin substrate 1 isoform Rac1 MGC111543, Ras-related C3 botulinum toxin substrate 2, Ras-related C3 botulinum toxin substrate 2, Rho family small GTP binding protein Rac 2, Rho family small GTP binding protein Rac1, Rho family small GTP binding protein Rac2, rho family small GTP binding protein Rac3, RP23-84C12.18, Small G protein, TC 25, ATGA0589-10 µg, ATGA0589-20 µg, ATGA05

References & Citations

Ridley AJ. (2006) . Trends Cell Biol. 16 (10) :522-529.; ; Maldonado MDM., et al. (2018) . Cancer Res. 15;78 (12) :3101-3111.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_002863

Uniprot Accession Number

P15153

WB Description

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human Rac 1 protein; ; Lane 2.: Recombinant human Rac 2 protein; ; Lane 3.: Recombinant human Rac 3 proteinThe cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate{ATGA0589-WB2.jpg}

FACS Description

Flow cytometry analysis of Rac1,2,3 in Jurkat cells. The cell was stained with ATGA0589 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .