Human IFN-beta 1/IFNB1 antibody

Interferon beta 1, also known as IFN-beta, is produced mainly by fibroblasts and some epithelial cell types. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. It also increases the expression of HLA class I antigens and blocks the expression of HLA class II antigens stimulated by IFN-gamma. Interferon beta 1 can be used for topic treatment of condylomata acuminata. Some studies suggest that Interferon beta 1 tends to prevent disease activity in patients with multiple sclerosis

Product Specifications

Product Name Alternative

Ifb, IFN-beta, IFNB, Interferon beta 1, Interferon beta, Fibroblast interferon

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human IFN-beta (22-187aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4F8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

IFN-b, ATGA0587-10 µg, ATGA0587-20 µg, ATGA0587-50 µg, ATGA0587-100 µg, ATGA0587-250 µg, ATGA0587-500 µg, ATGA0587-1 mg, ATGA0587-10, ATGA0587-20, ATGA0587-50, ATGA0587-100, ATGA0587-250, ATGA0587-500, ATGA0587-1

References & Citations

Fierlbeck G, et al. (1992) Hautarzt. 43 (1) :16-21.; ; Panitch HS. (1992) Drugs. 44 (6) :946-62.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_002167

Uniprot Accession Number

P01574

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IFN-beta antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Jurkat cell lysate;

IF Description

ICC/IF analysis of IFN-beta in Hela cells. The cell was stained with ATGA0587 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .