Human BHMT antibody

Betaine homocysteine methyltransferase, Betaine homocysteine S methyltransferase, Betaine homocysteine S methyltransferase 1, Betaine-homocysteine methyltransferase, Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase, Betaine-homocysteine S-methyltransferase 1, BHMT, BHMT (Betaine-homocysteine methyltransferase), BHMT1, EC 2.1.1.5, ATGA0584-10 µg, ATGA0584-20 µg, ATGA0584-50 µg, ATGA0584-100 µg, ATGA0584-250 µg, ATGA0584-500 µg, ATGA0584-1 mg, ATGA0584-010, ATGA0584-020, ATGA0584-050, ATGA0584-100, ATGA0584-250, ATGA0584-500, ATGA0584-01M

Mckeever MP et al., (1991) Clin Sci 81 (4) :551-556.; ; Norihiko F et al., (2001) Biochem J 129:313-320.; ; Francisco G et al., (2005) Biochem J 391:589-599.; ; Catherine M et al., (2005) Biochem J 392:443-448.; ; Janet D. S et al., (2006) Biochem J 395:363-371.

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Liver Tissue lysate

Flow cytometry analysis of BHMT in Hep3B cells. The cell was stained with ATGA0584 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of BHMT in Hep3B cells. The cell was stained with ATGA0584 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .