Human ACAT2 antibody

Acetyl-CoA acetyltransferase, cytosolic, also known as cytosolic acetoacetyl-CoA thiolase, is an enzyme that inhumans is encoded by the ACAT (acetyl-Coenzyme A acetyltransferase 2) gene. ACAT2 is an acetyl-CoAC-acetyltransferase enzyme, plays a role in regulating lipid metabolism. It catalyzes the synthesis ofacetoacetyl-CoA from two acetyl-CoA molecules, which is later converted into steroids.

Product Specifications

Product Name Alternative

Acetyl-CoA acetyltransferase cytosolic, ACTL, Acetyl-CoA acetyltransferase, cytosolic

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ACAT2 (1-397aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT55D5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ACAT2, ATGA0579-10 µg, ATGA0579-20 µg, ATGA0579-50 µg, ATGA0579-100 µg, ATGA0579-250 µg, ATGA0579-500 µg, ATGA0579-1 mg, ATGA0579-10, ATGA0579-20, ATGA0579-50, ATGA0579-100, ATGA0579-250, ATGA0579-500, ATGA0579-1

References & Citations

Shintani S., et al. (1999) Genetics. 152 (2) : 743-754.; ; Middleton B., et al. (1974) Biochem J. 139 (1) : 109-121.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_005882

Uniprot Accession Number

Q9BWD1

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: Ramos cell lysate; ; The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: ACAT2 Transfected 293T cell lysate; ; {ATGA0579-WB2.jpg}

IF Description

ICC/IF analysis of ACAT2 in HeLa cells. The cell was stained with ATGA0579 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .