Human PARP2 antibody

PARP2 is poly (ADP-ribosyl) transferase-like 2 protein, which contains a catalytic domain and is capable ofcatalyzing a poly (ADP-ribosyl) ation reaction. This protein has a catalytic domain which is homologous to thatof poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminalcatalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of thisprotein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targetingof the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Product Specifications

Product Name Alternative

Poly (ADP-ribose) polymerase 2, ADPRT2, ADPRTL2, ADPRTL3, ARTD2, pADPRT-2, PARP-2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PARP2 (233-583aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT29G4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PARP2, ATGA0578-10 µg, ATGA0578-20 µg, ATGA0578-50 µg, ATGA0578-100 µg, ATGA0578-250 µg, ATGA0578-500 µg, ATGA0578-1 mg, ATGA0578-10, ATGA0578-20, ATGA0578-50, ATGA0578-100, ATGA0578-250, ATGA0578-500, ATGA0578-1

References & Citations

Ame JC, Rolli V, Schreiber V et al. (1999) . J. Biol. Chem. 274 (25) : 17860-8.; ; Schreiber V, Ame JC, Dolle P et al. (2002) . J. Biol. Chem. 277 (25) : 23028-36.; ; Maeda Y, Hunter TC, Loudy DE et al. (2006) . J. Biol. Chem. 281 (14) : 9600-6.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenetics and Nuclear Signaling

NCBI Accession Number

NP_005475

Uniprot Accession Number

Q9UGN5

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: SW480 cell lysate; ; The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; {ATGA0578-WB2.jpg}The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: NIH3T3 cell lysate; ; Lane 1.: Raji cell lysate; ; {ATGA0578-WB3.jpg}

FACS Description

Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with ATGA0578 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with ATGA0578 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .