Human Lactate Dehydrogenase A/LDHA antibody

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1) . LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.

Product Specifications

Product Name Alternative

Lactate dehydrogenase A, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, PIG19, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human LDHA (1-332aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1A4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Lactate Dehydrogenase A, LDHA, ATGA0574-10 µg, ATGA0574-20 µg, ATGA0574-50 µg, ATGA0574-100 µg, ATGA0574-250 µg, ATGA0574-500 µg, ATGA0574-1 mg, ATGA0574-10, ATGA0574-20, ATGA0574-50, ATGA0574-100, ATGA0574-250, ATGA0574-500, ATGA0574-1

References & Citations

Le A, et al. (2010) Proc Natl Acad Sci U S A, 107 (5) :2037-42.; ; Xie H, et al. (2009) Mol Cancer Ther, 8 (3) :626-35.; ; Toyoda K, et al. (2010) J Bacteriol, 191 (13) :4251-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_005557

Uniprot Accession Number

P00338

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: A431 cell lysate;

IF Description

Flow cytometry analysis of Lactate Dehydrogenase A/LDHA in HeLa cells. The cell was stained with ATGA0574 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .