Human CTLA4 antibody

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that downregulates the immune system. CTLA4 is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Mutations in CTLA4 have been associated with insulin-dependent diabetes mellitus, Graves' disease, Hashimoto's thyroiditis, celiac disease and other autoimmune diseases.

Product Specifications

Product Name Alternative

Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CELIAC3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, IDDM12, CD, GSE

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CTLA4 (36-161aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4C8

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CTLA4, ATGA0572-10 µg, ATGA0572-20 µg, ATGA0572-50 µg, ATGA0572-100 µg, ATGA0572-250 µg, ATGA0572-500 µg, ATGA0572-1 mg, ATGA0572-10, ATGA0572-20, ATGA0572-50, ATGA0572-100, ATGA0572-250, ATGA0572-500, ATGA0572-1

References & Citations

Dariavach. P., et al. (1988) Eur J Immunol 18 (12) : 1901-1905.; ; Waterhouse. P., et al. (1995) Science 270 (5238) : 985-988.; ; Magistrelli. G., et al. (1999) Eur J Immunol 29 (11) : 3596-3602.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Stem cell

NCBI Accession Number

NP_005205

Uniprot Accession Number

P16410

WB Description

The tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTLA4 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse liver tissue lysate; ; Lane 2.: TF-1 cell lysate; ; Lane 3.: HepG2 cell lysate; ; Lane 4.: WiDr cell lysate;

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