Human Podoplanin antibody

Podoplanin (PDPN) is a small mucin-like transmembrane protein, widely expressed in various specialized celltypes throughout the body. This is a type-I integral membrane glycoprotein with diverse distribution in humantissues. The physiological function of this protein may be related to its mucin-type character.

Product Specifications

Product Name Alternative

TIA2, T1A, T1 ALPHA GENE, T1 alpha, Podoplanin, PDPN, PA2.26 antigen, OTTHuMP00000044504, OTTHuMP00000009640, OTS8, Lung type I cell membrane-associated glycoprotein isoform a, Lung type I cell membrane associated glycoprotein isoform a AGGRuS, Lung type I cell membrane associated glycoprotein, hT1alpha2, hT1alpha1, HT1A-1, GP40, Gp38, GP36, Glycoprotein 36 KD

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PDPN (1-206aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

500

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Podoplanin, ATGA0571-10 µg, ATGA0571-20 µg, ATGA0571-50 µg, ATGA0571-100 µg, ATGA0571-250 µg, ATGA0571-500 µg, ATGA0571-1 mg, ATGA0571-10, ATGA0571-20, ATGA0571-50, ATGA0571-100, ATGA0571-250, ATGA0571-500, ATGA0571-1

References & Citations

Wicki A., et al. (2006) Br J Cancer. 96 (1) :1-5.; ; Zimmer G., et al. (1999) Biochem J. 341 (Pt2) :277-84.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_006465

Uniprot Accession Number

Q86YL7

WB Description

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Podoplanin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human Podoplanin protein;

FACS Description

Flow cytometry analysis of Podoplanin in HeLa cells. The cell was stained with ATGA0571 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of Podoplanin in HeLa cells. The cell was stained with ATGA0571 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .