Human Tollip antibody

Toll interacting protein, also known as Tollip, is an inhibitory adaptor protein within the signaling pathwayof IL-1 and Toll-like receptors (TLR) . The Toll-like receptors are essential transmembrane signalingreceptors of the innate immune system that alert the host to the presence of a microbial invader. Tolliprecruits IRAk1 to the IL-1 receptor complex and inhibits IRAk1 phosphorylation and kinase activity.

Product Specifications

Product Name Alternative

Toll interacting protein, IL-1RAcPIP

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Tollip (61-274aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

K1B17

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Tollip, ATGA0568-10 µg, ATGA0568-20 µg, ATGA0568-50 µg, ATGA0568-100 µg, ATGA0568-250 µg, ATGA0568-500 µg, ATGA0568-1 mg, ATGA0568-10, ATGA0568-20, ATGA0568-50, ATGA0568-100, ATGA0568-250, ATGA0568-500, ATGA0568-1

References & Citations

Ciarrocchi A. et al., (2009) Published online. 4 (2) :e4404.; ; Didierlaurent A. et al., (2006) Mol Cell Biol. 26 (3) :735-742.; ; Opal S.M. et al., (2002) Crit Care. 6 (2) :125-136.; ; Burns K. et al., (2000) Nat Cell Biol. 2 (6) :346-351.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

NP_061882

Uniprot Accession Number

Q9H0E2

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tollip antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: HepG2 cell lysate;