Human GRP78/HSPA5 antibody
Binding immunoglobulin protein (BIP) also known as 78kDa glucose-regulated protein (GRP-78) or heatshock 70kDa protein 5 (HSPA5) is a HSP70 molecular chaperone located in the lumen of the endoplasmicreticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains themin a state competent for subsequent folding and oligomerization. BIP is also an essential component of thetranslocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrantproteins destined for degradation by the proteasome. BIP is an abundant protein under all growth conditions, butits synthesis is markedly induced under conditions that lead to the accumulation of unfolded polypeptides in theER.
Product Specifications
Product Name Alternative
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human BIP (20-650aa) purified from Baculo virus
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT3D2
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
References & Citations
Ting. J., et al. (1988) DNA 7 (4) : 275-86.; ; Hendershot. L.M., et al. (1994) Genomics 20 (2) : 281-284.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
Scientific Category
Cancer
NCBI Accession Number
NP_005338
Uniprot Accession Number
P11021
WB Description
IF Description
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