GAPDH antibody

Wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38 KD compone

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Chang Y, et al. Clinical impact of serum exosomal microRNA in liver fibrosis. (PLoS One. 2021) {https://pubmed.ncbi.nlm.nih.gov/34506494/}; ; Hah YS, et al. β-Sitosterol Attenuates Dexamethasone-Induced Muscle Atrophy via Regulating FoxO1-Dependent Signaling in C2C12 Cell and Mice Model. (Nutrients. 2022) {https://pubmed.ncbi.nlm.nih.gov/35889851/}; ; Hah YS, et al. Rutin Prevents Dexamethasone-Induced Muscle Loss in C2C12 Myotube and Mouse Model by Controlling FOXO3-Dependent Signaling. (Antioxidants. 2023) {https://pubmed.ncbi.nlm.nih.gov/36978887/}; ; Park SW, et al. Development of new tools to study membrane-anchored mammalian Atg8 proteins. (Autophagy. 2023) {https://pubmed.ncbi.nlm.nih.gov/36250672/}

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with ant-GAPDH antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: MCF7 cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: K562 cell lysate; ; Lane 5.: A431 cell lysate; ; Lane 6.: LNCap cell lysate; ; Lane 7.: Ramos cell lysateThe tissue lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probedwith anti-GAPDH antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Brain tissue lysate; ; Lane 2.: Mouse Spleen tissue lysate; ; Lane 3.: Mouse Eye tissue lysate; ; Lane 4.: Mouse Muscle tissue lysate{ATGA0559-WB2.jpg}The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRPand an ECL detection system.; ; Lane 1.: Anti-GAPDH monoclonal antibody (1:100,000) ; ; Lane 2.: Anti-GAPDH monoclonal antibody (1:50,000) ; ; Lane 3.: Anti-GAPDH monoclonal antibody (1:10,000) ; ; Lane 4.: Anti-GAPDH monoclonal antibody (1:1,000) ; ; {ATGA0559-WB3.jpg}

ICC/IF analysis of GAPDH in HeLa cells. The cell was stained with ATGA0559 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .