Human Troponin I1/TNNI1 antibody

Troponin I, slow skeletal muscle, also known as TNNI1, belongs to the troponin I family. The troponin I subfamily contains three genes: TNNI-skeletal-fast-twitch, TNNI-skeletal-slow-twitch, and TNNI-cardiac. The TNNI-fast and TNNI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TNNI-cardiac gene is expressed exclusively in cardiac muscle tissue. TNNI1 is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation.

Product Specifications

Product Name Alternative

Troponin I1 slow skeletal muscle, Troponin I, slow skeletal muscle, DKFZp451O223, SSTNI, TNN1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TNNI1 (1-187aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT36E7

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TNNI1, ATGA0558-10 µg, ATGA0558-20 µg, ATGA0558-50 µg, ATGA0558-100 µg, ATGA0558-250 µg, ATGA0558-500 µg, ATGA0558-1 mg, ATGA0558-10, ATGA0558-20, ATGA0558-50, ATGA0558-100, ATGA0558-250, ATGA0558-500, ATGA0558-1

References & Citations

Hunkeler NM, Kullman J, Murphy AM (1991) . Circ. Res. 69 (5) : 1409-14.; ; Bhavsar PK, Dhoot GK, Cumming DV, et al. (1992) . FEBS Lett. 292 (1-2) : 5-8.; ; Westfall MV, Borton AR (2003) . J. Biol. Chem. 278 (36) : 33694-700.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003272

Uniprot Accession Number

P19237

WB Description

The extracts of mouse muscle (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TNNI1 antibody (1:500~5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: TNNI1 mAb 1:500; ; Lane 2.: TNNI1 mAb 1:1000; ; Lane 3.: TNNI1 mAb 1:5000