Human GARS antibody

GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetase that charge tRNAswith their cognate amino acids. Defects in the gene encoding GlyRS is the cause of Charcot-Marie-Toothdisease type 2D (CMT2D), which is an autosomal dominant inherited disease characterized by severe weakness, atrophy and absence of deep tendon reflexes in the upper extremities. Defects in the GlyRS gene is also thecause of distal hereditary muscular neuropathy type V (HMN5), a disease similar to CMT2D, though the distalsensory involvement is less severe in HMN5 patients.

Product Specifications

Product Name Alternative

Glycyl-tRNA synthetase, CMT2D, DSMAV, GlyRS, HMN5, SMAD1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GARS (43-289aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT4E10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GARS, ATGA0557-10 µg, ATGA0557-20 µg, ATGA0557-50 µg, ATGA0557-100 µg, ATGA0557-250 µg, ATGA0557-500 µg, ATGA0557-1 mg, ATGA0557-10, ATGA0557-20, ATGA0557-50, ATGA0557-100, ATGA0557-250, ATGA0557-500, ATGA0557-1

References & Citations

Xie. W. (2007) Proc Natl Acad Sci U S A. 104: 9976-9981.; ; Antonellis. A. (2003) AM J Hum Genet 72: 1293-1299.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenetics and Nuclear Signaling

NCBI Accession Number

NP_002038

Uniprot Accession Number

P41250

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GARS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: U87MG cell lysate; ; Lane 5.: A431 cell lysate;

IF Description

ICC/IF analysis of GARS in HeLa cells. The cell was stained with ATGA0557 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .