Human visfatin/NAMPT antibody

Visfatin is pre-B cell colony-enhancing factor 1 (PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor.

Product Specifications

Product Name Alternative

Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Pre-B cell-enhancing factor, PBEF, PBEF1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PBEF/Visfatin (1-491aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

4D5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PBEF, Visfatin, ATGA0556-10 µg, ATGA0556-20 µg, ATGA0556-50 µg, ATGA0556-100 µg, ATGA0556-250 µg, ATGA0556-500 µg, ATGA0556-1 mg, ATGA0556-10, ATGA0556-20, ATGA0556-50, ATGA0556-100, ATGA0556-250, ATGA0556-500, ATGA0556-1

References & Citations

Stephens JM, et al., (2006) Curr Opin Lipidol. 17:128-31; ; Hug C, et al., (2005) Science. 307:366-367

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_005737

Uniprot Accession Number

P43490

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PBEF/Visfatin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: SW480 cell lysate;

IF Description

ICC/IF analysis of PBEF/Visfatin in HeLa cells. The cell was stained with ATGA0556 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .