Human MYL4 antibody

Myosin light chain 4 is a protein that in humans is encoded by the MYL4 gene. Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

Product Specifications

Product Name Alternative

Myosin light chain 4, Myosin, light chain 4, ALC1, AMLC, GT1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MYL4 (1-197aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT4E8

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MYL4, ATGA0552-10 µg, ATGA0552-20 µg, ATGA0552-50 µg, ATGA0552-100 µg, ATGA0552-250 µg, ATGA0552-500 µg, ATGA0552-1 mg, ATGA0552-10, ATGA0552-20, ATGA0552-50, ATGA0552-100, ATGA0552-250, ATGA0552-500, ATGA0552-1

References & Citations

Kurabayashi, M., et al. (1998) J Biol Chem 263 (27) : 13930-6.; ; Rotter, M., et al. (1991) Nucleic Acids Res 19 (7) : 1497-504.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001002841

Uniprot Accession Number

P12829

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL4 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: K-562 cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: Jurkat cell lysate; ; Lane 6.: 293T cell lysate; ; Lane 7.: LnCap cell lysate