Human Crystallin alpha A/CRYAA antibody

Alpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively.alpha-crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP) . They actas molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weightaggregates. These heterogeneous aggregates consist of 30-40 subunits of the alpha-A and alpha-B subunitshave a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and theparticipation in the intracellular architecture.

Product Specifications

Product Name Alternative

Acry-1, Alpha crystallin A chain, CRYA1, CRYAA, Crystallin alpha 1, Crystallin alpha A, Heat shock protein beta 4, HSPB 4, HspB4, Zonular Central Nuclear Cataract

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human AlphaA Crystallin/CRYAA (1-173aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

C9F2

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

A A Crystallin, Acry-1, Alpha crystallin A chain, Alpha-crystallin A chain, CRYA 1, CRYA1, CRYAA, Crystallin Alpha 1, Crystallin alpha A, Heat shock protein beta 4, Heat shock protein beta-4, HSPB 4, HspB4, Zonular Central Nuclear Cataract, ATGA0550-10 µg, ATGA0550-20 µg, ATGA0550-50 µg, ATGA0550-100 µg, ATGA0550-250 µg, ATGA0550-500 µg, ATGA0550-1 mg, ATGA0550-010, ATGA0550-020, ATGA0550-050, ATGA0550-100, ATGA0550-250, ATGA0550-500, ATGA0550-01M

References & Citations

Fujii N., et al., (2003) Mol. Vis. 9: 915-22.; ; Laksanalamai P., et al., (2004) Extremophiles 8: 1-11.;

Other References

Oh JY, et al. Identification of the HSPB4/TLR2/NF-κB axis in macrophage as a therapeutic target for sterile inflammation of the cornea. (EMBO Mol Med. 2012) {https://pubmed.ncbi.nlm.nih.gov/22359280/}; ; Fan Q, et al. Identification of proteins that interact with alpha A-crystallin using a human proteome microarray. (Mol Vis. 2014) {https://pubmed.ncbi.nlm.nih.gov/24453475/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_000385

Uniprot Accession Number

P02489

WB Description

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AlphaA Crystallin/CRYAA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human AlphaA Crystallin/CRYAA protein; ; Lane 2.: Recombinant human AlphaB Crystallin/CRYAB protein; ; The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AlphaA Crystallin/CRYAA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: AlphaA Crystallin/CRYAA Transfected 293T cell lysate; ; {ATGA0550-WB2.jpg}