Human Casein kinase 1 alpha/CSNK1A1 antibody

Caseine Kinase 1 alpha belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily. CK1 isoforms (alpha, beta, gamma, delta, epsilon) and their splice variants are involved in diverse cellular processes including membrane trafficking, circadian rhythm, cell cycle progression, chromosome segregation, apoptosis and cellular differentiation. Perhaps it can phosphorylate a large number of proteins and is involved in Wnt signaling where it phosphorylates CTNNB1 on Ser45. Caseine kinase 1 alpha also interacts with the Axin complex.

Product Specifications

Product Name Alternative

Casein kinase 1 alpha 1, CK1, CK1a, CK1alpha, CKIa, CKIalpha, Clock regulator kinase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Casein Kinase 1 alpha (1-337aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2E2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Casein Kinase1 a, ATGA0549-10 µg, ATGA0549-20 µg, ATGA0549-50 µg, ATGA0549-100 µg, ATGA0549-250 µg, ATGA0549-500 µg, ATGA0549-1 mg, ATGA0549-10, ATGA0549-20, ATGA0549-50, ATGA0549-100, ATGA0549-250, ATGA0549-500, ATGA0549-1

References & Citations

Knippschild U, et al., (2005) Onkologie. 28 (10) :508-14.; ; Katoh M., (2007) Clin Cancer Res. 13 (14) :4042-5.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001883

Uniprot Accession Number

P48729

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Casein kinase 1 alpha antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: MCF7 cell lysate;

IF Description

ICC/IF analysis of Casein kinase 1 alpha in HeLa cells. The cell was stained with ATGA0549 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .