Human SUMO 2/3 antibody

SUMO (small ubiquitin-related modifier) is the best-characterized member of a growing family ofubiquitin-related proteins. 8-11 kDa that covalently modify various intracellular proteins. It resemblesubiquitin in its structure, its ability to be ligated to other proteins. SUMO regulates cellular function of avariety of target proteins. SUMO proteins are expressed as their precursor forms. Cleavage of the residuesafter the GG region of these precursors by SUMO-specific proteases in maturation is a prerequisite forsubsequent sumoylation. Notably, SUMO2 and SUMO3 precursors have 96% sequence identity, and recentstudies have shown protein substrates conjugated with SUMO2 or SUMO3 have similar, if not identical, biological consequences.

Product Specifications

Product Name Alternative

Ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, SuMO3, SuMO2, SMT3H2, SMT3H1, SMT3B, SMT3A, SMT3 suppressor of mif two 3 homolog 2, SMT3 homolog 2, SMT 3B, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), Small ubiquitin related modifier 2, Small ubiquitin like modifier 2, Sentrin2, MGC117191, HSMT3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SUMO2 (1-93aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT10F1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

HSMT 3, HSMT3, MGC117191, Sentrin 2, Sentrin2, Sentrin-2, Small ubiquitin like modifier 2, Small ubiquitin related modifier 2, Small ubiquitin-related modifier 2, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), SMT 3B, SMT3 homolog 2, SMT3 suppressor of mif two 3 homolog 2, SMT3A, SMT3B, SMT3H1, SMT3H2, SuMO 3, SuMO2, SuMO-2, SuMO3, SuMO-3, ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, ATGA0547-10 µg, ATGA0547-20 µg, ATGA0547-50 µg, ATGA0547-100 µg, ATGA0547-250 µg, ATGA0547-500 µg, ATGA0547-1 mg, ATGA0547-010, ATGA0547-020, ATGA0547-050, ATGA0547-100, ATGA0547-250, ATGA0547-500, ATGA0547-01M

References & Citations

Tatsuya Ii, et al. (2007) DNA Repair (Amst), 6 (11) : 1679-1691.; ; Zheng Xu, et al. (2005) Biochem J, 386 (Pt 2) : 325-330.; ; Melchior F. (2000) Annu Rev Cell Dev Biol, 16: 591-626.;

Other References

Ham JH, et al. An Immunohistochemical Study on the Expression of SUMO-2/3 in the Colorectal Carcinoma. (Soonchunhyang Med Sci. 2012) {https://jsms.sch.ac.kr/journal/view.php?year=2012&vol=18&no=2&spage=95}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenetics and Nuclear Signaling

NCBI Accession Number

NP_008868

Uniprot Accession Number

P61956

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: K562 cell lysate; ; Lane 4.: HL-60 cell lysateThe recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human SUMO1 protein; ; Lane 2.: Recombinant human SUMO2 protein; ; Lane 3.: Recombinant human SUMO3 protein; ; {ATGA0547-WB2.jpg}

FACS Description

Flow cytometry analysis of SUMO2/3 in Jurkat cells. The cell was stained with ATGA0547 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of SUMO2/3 in HeLa cells. The cell was stained with ATGA0547 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .