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Human STAT1 antibody

STAT1, also known as STAT91, is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 is activated by peptide hormones, growth factors and cytokines, particularly cytokines of the interferon family.

Product Specifications

Product Name Alternative

Signal transducer and activator of transcription 1 91kDa, ISGF-3, STAT91, Transcription factor ISGF-3 components p91/p84, Signal transducer and activator of transcription 1, 91kDa

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human STAT1 (1-712aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1F4

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

STAT1, ATGA0546-10 µg, ATGA0546-20 µg, ATGA0546-50 µg, ATGA0546-100 µg, ATGA0546-250 µg, ATGA0546-500 µg, ATGA0546-1 mg, ATGA0546-10, ATGA0546-20, ATGA0546-50, ATGA0546-100, ATGA0546-250, ATGA0546-500, ATGA0546-1

References & Citations

Farlik M., et al. (2010) Immunity. 33 (1) :25-34.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_009330

Uniprot Accession Number

P42224

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STAT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: A431 cell lysate;
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