His-tag antibody

A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and by the trademarked name His-tag.

Product Specifications

Product Name Alternative

6 His epitope tag antibody, Hexa His tag antibody, HHHHHH epitope tag antibody, HHHHHH tag antibody, His tag antibody, Polyhistidine Tag antibody

Host

Mouse

Immunogen

6x His synthetic peptide

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1D6

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

His tag, ATGA0544-10 µg, ATGA0544-20 µg, ATGA0544-50 µg, ATGA0544-100 µg, ATGA0544-250 µg, ATGA0544-500 µg, ATGA0544-1 mg, ATGA0544-10, ATGA0544-20, ATGA0544-50, ATGA0544-100, ATGA0544-250, ATGA0544-500, ATGA0544-1

References & Citations

O Hare, Simura, Gibson; et al. (2006) . Biomolecular Methods. 38 (22) : 873-885.; ; Li Y et al. from the Mariana Trench. Mar Drugs 17: (2019) .; ; Andersen, Kasper R.; Leksa, Nina C.; Schwartz, Thomas U. (2013) .;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-His tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1: OTUB2-His tag Transfected 293T cell lysate; ; Lane 2: GAD-His tag Transfected 293T cell lysate; ; The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-His tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1: Recombinant human TREM1-His tag protein; ; Lane 2: Recombinant human CD34-His tag protein; ; Lane 3: Recombinant human ATG3-His tag protein; ; {2004010491789039.jpg}