What is the catalog number for Human Caveolin-1 antibody?

The catalog number for Human Caveolin-1 antibody is ATGA0540-050.

What sizes are available for Human Caveolin-1 antibody?

Human Caveolin-1 antibody is available in the following size(s): 50 µL.

Is Human Caveolin-1 antibody in stock?

Human Caveolin-1 antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Human Caveolin-1 antibody

The identification of caveolin-1 (CAV1) as the main structural component of caveolae, together with the finding that CAV1 might serve as a molecular organizer for membrane multiprotein complexes involved in cellular traffic, endo-and transcytosis, cell adhesion and signal transduction prompted a new impulse in the research on these intracellular organells. One of the properties of CAV1 was its insolubility in cold non-ionic detergents together with apical markers in epithelial cell. Also, cholesterol is essential for caveolae formation and maintenance, and caveolae structure is highly sensitive to cholesterol depletion or treatment with cholesterol binding drugs. CAV1 tightly and specifically binds free cholesterol and artificial cholesterol containing vesicles.

Product Specifications

Product Name Alternative

VIP 21, CAV, CAV 1, CAV1, caveolae protein 22 kD, caveolin 1 alpha isoform, caveolin 1 beta isoform, Caveolin 1 caveolae protein 22kDa, Caveolin1, cell growth-inhibiting protein 32, MSTP085, OTTHUMP00000025031, VIP 21, VIP21

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CAV1 (1-104aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT4C1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Caveolin1, ATGA0540-10 µg, ATGA0540-20 µg, ATGA0540-50 µg, ATGA0540-100 µg, ATGA0540-250 µg, ATGA0540-500 µg, ATGA0540-1 mg, ATGA0540-10, ATGA0540-20, ATGA0540-50, ATGA0540-100, ATGA0540-250, ATGA0540-500, ATGA0540-1

References & Citations

Van Meer, G., et al. (2008) Nat Rev Mol Cell Biol. 9: 112-124; ; Todeschini, R.A. et al. (2008) Biochim Biophy Acta 1780: 421-433; ; Lajoie, P et al. (2007) J Cell Mol Med 11: 644-653

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_001744

Uniprot Accession Number

Q03135

WB Description

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAV1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse heart tissue lysate

IF Description

ICC/IF analysis of CAV1 in A549 cells. The cell was stained with ATGA0540 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .