Human Arginase 2/ARG2 antibody

Arginase 2 catalyzes the same reaction as arginase 1, but differs in its tissue specificity and subcellularlocation. Specifically, arginase 2 localizes to the mitochondria. Arginase 2 is expressed in non-hepatictissues, with the highest levels of expression in the kidneys, but, unlike arginase 1, is not expressed inliver.

Product Specifications

Product Name Alternative

Arginase-2 type II, Kidney arginase, Non-hepatic arginase, Arginase-2, type II Arginase IImitochondrial, Kidney type arginase, Non hepatic arginase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ARG2 (23-354aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT7F8

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Arginase2, ARG2, ATGA0539-10 µg, ATGA0539-20 µg, ATGA0539-50 µg, ATGA0539-100 µg, ATGA0539-250 µg, ATGA0539-500 µg, ATGA0539-1 mg, ATGA0539-10, ATGA0539-20, ATGA0539-50, ATGA0539-100, ATGA0539-250, ATGA0539-500, ATGA0539-1

References & Citations

Watts JA., et al. (2011) Pulm Pharmacol Ther. 24 (4) : 407-413.; ; Tadie JM., et al. (2008) Am J Physiol Lung Cell Mol Physiol. 294 (3) : 489-497.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenetics and Nuclear Signaling

NCBI Accession Number

NP_001163

Uniprot Accession Number

P78540

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARG2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: SW480 cell lysate;