Human MafK antibody

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locuscontrol regions (LCRs) . LCRs are associated with powerful enhancer activity that is mediated by thetranscription factor NFE2 (nuclear factor erythroid-2) . NFE2 DNA-binding activity consists of aheterodimer containing an 18-kD Maf protein (MafF, MafG or MafK) and p45. Since Maf homodimers lack aputative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize amongthemselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerizewith Fos and may act as competitive repressors of the NF-E2 transcription factor. The MafK of Maf proteinsis primarily expressed during development in mesenchymal and hematopoietic cells and neurons.

Product Specifications

Product Name Alternative

Transcription factor MafK, NFE2u, P18

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MAFK (1-156aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2F7

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MafK, ATGA0538-10 µg, ATGA0538-20 µg, ATGA0538-50 µg, ATGA0538-100 µg, ATGA0538-250 µg, ATGA0538-500 µg, ATGA0538-1 mg, ATGA0538-10, ATGA0538-20, ATGA0538-50, ATGA0538-100, ATGA0538-250, ATGA0538-500, ATGA0538-1

References & Citations

Oyake, T., et al. (1996) Mol Cell Biol 16 (11) : 6083-95.; ; Toki, T., et al. (1997) Oncogene 14: 1901-1910.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenetics and Nuclear Signaling

NCBI Accession Number

NP_002351

Uniprot Accession Number

O60675

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MafK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MafK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human MafK protein; ; Lane 2.: Recombinant human MafG protein; ; Lane 3.: Recombinant human MafF protein; ; {2004010481853669.jpg}