Influenza A H3N2 Hemagglutinin antibody

Influenza A virus subtype H3N2, an enveloped virus of the Orthomyxoviridae family, is a respiratory infection in birds and mammals, and is an important cause of human influenza. In birds, humans, and pigs, the virus has mutated into many strains. Its derives from the forms of the two kinds of proteins on the surface of its coat, hemagglutinin (HA) and neuraminidase (NA) . Influenza A viruses are further classified into 16HA (H1-H16) and 9NA (N1-N9) serotypes based on the antigenic characteristics of HA and NA envelop glycoprotein. The extent of infection into host organisms are determined by HA, which interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues.

Product Specifications

Product Name Alternative

Hemagglutinin, Influenza A virus (A/Perth/16/2009 (H3N2) segment 4 hemagglutinin (HA) gene

Host

Mouse

Antigen Species

Human

Reactivity

Influenza A

Immunogen

Recombinant Influenza A H3N2/HA1 (17-345aa) purified from Baculovirus

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1B7

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-G affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

H3N2, HA, ATGA0535-10 µg, ATGA0535-20 µg, ATGA0535-50 µg, ATGA0535-100 µg, ATGA0535-250 µg, ATGA0535-500 µg, ATGA0535-1 mg, ATGA0535-10, ATGA0535-20, ATGA0535-50, ATGA0535-100, ATGA0535-250, ATGA0535-500, ATGA0535-1

References & Citations

Thomas H., et al. (2011) Ann N Y Acad Sci 1217: 178-190.; ; Wilson I.A., Cox N.J. (1990) Annu Rev Immunol 8: 737-771.; ; Cheng X., et al. (2012) Virology 432 (1) : 91-98.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Virus

NCBI Accession Number

ACS71642.1

Uniprot Accession Number

C6KNH7

WB Description

The recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Influenza A H3N2/HA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Influenza A H1N1/HA protein; ; Lane 2.: Recombinant Influenza A H5N2/HA protein; ; Lane 3.: Recombinant Influenza A H3N2/HA protein;