Human MYL2 antibody

Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Product Specifications

Product Name Alternative

Slow cardiac myosin regulatory light chain 2, MLC2, CMH10, DKFZp779C0562, Slow cardiac myosin regulatory light chain 2, MYL2, Slow cardiac myosin regulatory light chain 2 Cardiac myosin light chain-2, MLC 2v, MYL 2, Cardiac ventricular myosin light chain 2, RLC of myosin, Myosin light chain 2 regulatory cardiac slow, Myosin light polypeptide 2 regulatory cardiac slow, Myosin regulatory light chain 2 ventricular cardiac muscle isoform, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform, Regulatory light chain of myosin

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human MYL2 (1-166aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT3B2

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

MYL2, ATGA0531-10 µg, ATGA0531-20 µg, ATGA0531-50 µg, ATGA0531-100 µg, ATGA0531-250 µg, ATGA0531-500 µg, ATGA0531-1 mg, ATGA0531-10, ATGA0531-20, ATGA0531-50, ATGA0531-100, ATGA0531-250, ATGA0531-500, ATGA0531-1

References & Citations

Macera MJ, et al., (1992) Genomics. 13 (3) :829-31.; ; Poetter K, et al., (1996) Nat Genet. 13 (1) :63-9.

Other References

Sapp V, et al. Genome-wide CRISPR/Cas9 screening in human iPS derived cardiomyocytes uncovers novel mediators of doxorubicin cardiotoxicity. (Sci Rep. 2021) {https://pubmed.ncbi.nlm.nih.gov/34230586/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_000423

Uniprot Accession Number

P10916

WB Description

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Heart Tissue lysate

FACS Description

Flow cytometry analysis of MYL2 in A431 cells. The cell was stained with ATGA0531 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .