Human TLR7 antibody

TLR7 is one of toll-like receptors which are type I transmembrane proteins involved in innate immunity byrecognizing microbial conserved structures. These proteins are characterized by multiple copies of leucine richrepeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain, and act through adaptormolecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Human TLR7 (hTLR7) is predominantly expressed in lung, placenta and spleen and hTLR7, 8 genes are located on the sexchromosome X. hTLR7 may play an important role in detecting and combating viral infections.

Product Specifications

Product Name Alternative

Toll-like receptor 7, PRO285, UNQ248, tlr 7

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TLR7 (451-500aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

4F4

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TLR7, ATGA0530-10 µg, ATGA0530-20 µg, ATGA0530-50 µg, ATGA0530-100 µg, ATGA0530-250 µg, ATGA0530-500 µg, ATGA0530-1 mg, ATGA0530-10, ATGA0530-20, ATGA0530-50, ATGA0530-100, ATGA0530-250, ATGA0530-500, ATGA0530-1

References & Citations

Lee J., et al. (2003) Proc. Natl. Acad. Sci. USA. 100:6646-6651.; ; Crozat K., et al. (2004) Proc. Natl. Acad. Sci. USA. 101:6835-6836.; ; Muzio M., et al. (1998) J. Exp. Med. 187:2097-2101.; ; Horng T., et al. (2002) Nature 420:329-333.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenetics and Nuclear Signaling

NCBI Accession Number

NP_057646

Uniprot Accession Number

Q9NYK1

WB Description

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TLR7 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human TLR7 protein;

FACS Description

Flow cytometry analysis of TLR7 in THP-1 cells. The cell was stained with ATGA0530 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .