Human RASSF1A antibody

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.

Product Specifications

Product Name Alternative

RASSF1 (Ras association (RalGDS/AF-6) domain family 1),123F2, RDA32, NORE2A, RASSF1A, REH3P21, Ras association (RalGDS/AF-6) domain family member 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human RASSF1A (1-340aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT3F3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

RASSF1A, ATGA0528-10 µg, ATGA0528-20 µg, ATGA0528-50 µg, ATGA0528-100 µg, ATGA0528-250 µg, ATGA0528-500 µg, ATGA0528-1 mg, ATGA0528-10, ATGA0528-20, ATGA0528-50, ATGA0528-100, ATGA0528-250, ATGA0528-500, ATGA0528-1

References & Citations

Agathanggelou A, et al., (2005) Cancer Res. 65, 3497-3508.; ; Lerman MI. et. al. (2000) Cancer Res. 60, 6116-6133. ; ; Strunnikova M. et al., (2005) Mol Cell Biol. 25, 3923-3933. ; ; Dammann R. et al., (2005) Histol Histopathol. 20, 645-663. ; ; Rossa W.k, et al., (2007) American Journal of pathology. 170, 941-950.

Other References

Li W, et al. Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation. (Cell Death Differ. 2019) {https://pubmed.ncbi.nlm.nih.gov/30315205/}; ; Guo M, et al. RASSF1-AS1, an antisense lncRNA of RASSF1A, inhibits the translation of RASSF1A to exacerbate cardiac fibrosis in mice. (Cell Biol Int. 2019) {https://pubmed.ncbi.nlm.nih.gov/30571844/}; ; Dabral S, et al. A RASSF1A-HIF1α loop drives Warburg effect in cancer and pulmonary hypertension. (Nat Commun. 2019) {https://pubmed.ncbi.nlm.nih.gov/31086178/}; ; Francisco J, et al. The tumor suppressor RASSF1A modulates inflammation and injury in the reperfused murine myocardium. (J Biol Chem. 2019) {https://pubmed.ncbi.nlm.nih.gov/31311858/}; ; Chen J, et al. 5-Aza-CdR Regulates RASSF1A By Inhibiting DNMT1 To Affect Colon Cancer Cell Proliferation, Migration And Apoptosis. Cancer Manag Res. 2019{https://pubmed.ncbi.nlm.nih.gov/31807076/}; ; Slemmons KK, et al. Targeting Hippo-Dependent and Hippo-Independent YAP1 Signaling for the Treatment of Childhood Rhabdomyosarcoma. (Cancer Res. 2020) {https://pubmed.ncbi.nlm.nih.gov/32354737/}; ; Dhanaraman T, et al. RASSF effectors couple diverse RAS subfamily GTPases to the Hippo pathway. Sci Signal. 2020{https://pubmed.ncbi.nlm.nih.gov/33051258/}; ; Jeon HJ, Oh JS. RASSF1A Regulates Spindle Organization by Modulating Tubulin Acetylation via SIRT2 and HDAC6 in Mouse Oocytes. (Front Cell Dev Biol. 2020) {https://pubmed.ncbi.nlm.nih.gov/33195286/}; ; Jia J, et al. RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein. (J Cell Mol Med. 2021) {https://pubmed.ncbi.nlm.nih.gov/33960627/}; ; Roßwag S, et al. RASSF1A-Mediated Suppression of Estrogen Receptor Alpha (ERα) -Driven Breast Cancer Cell Growth Depends on the Hippo-Kinases LATS1 and 2. (Cells. 2021) {https://pubmed.ncbi.nlm.nih.gov/34831091/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_009113

Uniprot Accession Number

Q9NS23

WB Description

The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: RASSF1A Transfected 293T cell lysate{2004010467743607.jpg}

IF Description

ICC/IF analysis of RASSF1A in HeLa cells. The cell was stained with ATGA0528 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .