Human CD158f/KIR2DL5A antibody

KIR/CD158, also known as killer cell immunoglobulin-like receptor 2DL5A, is a type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. It is detected on the cell surface as a monomer that, upon tyrosine phosphorylation, recruits the Srchomology region 2-containing protein tyrosine phosphatase-2, It is thus an inhibitory receptor gathering a combination of genetic, structural, and functional features unique among KIR, which suggests that it plays a specialized role in innate immunity.

Product Specifications

Product Name Alternative

Killer cell immunoglobulin like receptor two Ig domains and long cytoplasmic tail 5A, Killer cell immunoglobulin-like receptor 2DL5A, KIR2DL5A, CD158F, KIR2DL5A.1, KIR2DL5A.3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human KIR/CD158 (22-238aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT11C11

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

KIR, CD158, ATGA0525-10 µg, ATGA0525-20 µg, ATGA0525-50 µg, ATGA0525-100 µg, ATGA0525-250 µg, ATGA0525-500 µg, ATGA0525-1 mg, ATGA0525-10, ATGA0525-20, ATGA0525-50, ATGA0525-100, ATGA0525-250, ATGA0525-500, ATGA0525-1

References & Citations

Vilches, C., et al. (2000) J. Immunol. 164: 5797-5804.; ; Elfishawi SM., et al. (2017) Hum. Immunol. 78 (11-12), 684-691.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Other

NCBI Accession Number

NP_065396

Uniprot Accession Number

Q8N109

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR/CD158 antibody (1:10000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: MCF7 cell lysate; ; Lane 3.: HepG2 cell lysate; ; Lane 4.: K562 cell lysate

FACS Description

Flow cytometry analysis of KIR/CD158 in PBMC. The cell was stained with ATGA0525 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .