Human BAK antibody

BAK1 encodes an receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 expression is associated with the progression of Prostate cancer (CaP) . BAK1 appears to function in distinct receptor-signaling complexes to integrate multiple microbe-associated molecular pattern (MAMP) perception into downstream-signaling events. BAK1 can serve multiple purposes to simultaneously modulate MAMP-receptor complexes, BR signaling, and cell death for the benefit of bacterial infection, life style, and fitness.

Product Specifications

Product Name Alternative

BCL2-antagonist/killer 1, Apoptosis regulator BAK, BAK, BAK-LIKE, Bcl-2 homologous antagonist/killer, Bcl2-L-7, BAK-1, BAK 1, BAK like, Bak NT, BAK1, Bcl 2 homologous antagonist/killer, Bcl 2 like 7 protein, Bcl2 homologous antagonist killer, Bcl2 like 7 Protein, BCL2-antagonist/killer 1, BCL2L7, CDN 1, CDN1, Cell death inhibitor 1, MGC117255, MGC3887, NBak, Pro apoptotic protein BAK

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BAK (29-187aa) purified from E.coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT38E2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

BAK, ATGA0523-10 µg, ATGA0523-20 µg, ATGA0523-50 µg, ATGA0523-100 µg, ATGA0523-250 µg, ATGA0523-500 µg, ATGA0523-1 mg, ATGA0523-10, ATGA0523-20, ATGA0523-50, ATGA0523-100, ATGA0523-250, ATGA0523-500, ATGA0523-1

References & Citations

Birgit Kemmerling, et al. (2008) Plant Signal Behav, 3 (2) : 116-118.; ; Libo Shan, et al. (2008) Cell Host Microbe, 4 (1) : 17-27.; ; Xu-Bao Shi, et al. (2007) Proc Natl Acad Sci U S A, 104 (50) : 19983-19988.

Other References

Singh A, et al. Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen. (Front Immunol. 2017) {https://pubmed.ncbi.nlm.nih.gov/28289414/}; ; Mebratu YA, et al. Bik reduces hyperplastic cells by increasing Bak and activating DAPk1 to juxtapose ER and mitochondria. (Nat Commun. 2017) {https://pubmed.ncbi.nlm.nih.gov/28986568/}; ; Liu P, et al. Downregulation of microRNA-125a is involved in intervertebral disc degeneration by targeting pro-apoptotic Bcl-2 antagonist killer 1. (Iran J Basic Med Sci. 2017) {https://pubmed.ncbi.nlm.nih.gov/29299204/}; ; Loo LSW, et al. BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells. (Cell Death Dis. 2020) {https://pubmed.ncbi.nlm.nih.gov/32424151/}; ; Shen J, et al. TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy. (Diabetol Metab Syndr. 2022) {https://pubmed.ncbi.nlm.nih.gov/35120573/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_001179

Uniprot Accession Number

Q16611

WB Description

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : 293T cell lysate; ; Lane 2 : HeLa cell lysate; ; Lane 3 : A431 cell lysate; ; Lane 4 : A549 cell lysate; ; Lane 5 : Jurkat cell lysate ; ; Lane 6 : MCF7 cell lysate ; ; Lane 7 : PC3 cell lysate

FACS Description

Flow cytometry analysis of BAK in HeLa cells. The cell was stained with ATGA0523 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of BAK in HeLa cells. The cell was stained with ATGA0523 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .