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Human FABP1/L-FABP antibody

FABP1 (Fatty acid binding protein1) encodes the fatty acid binding protein found in liver. FABP1 is composed of ten antiparallelbeta strands that form a barrel with a bigger binding pocket than the other FABPs allowing it to accommodate two fatty acid. This protein binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm; it may be involved in intracellular lipid transport and metabolism.

Product Specifications

Product Name Alternative

Fatty acid binding protein 1, Fatty acid-binding protein, liver, FABPL, L-FABP, FABP1, Fatty acid binding protein 1, fatty acid binding protein 1 liver

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FABP1/L-FABP (1-127aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

2G4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

FABP1, L-FABP, ATGA0521-10 µg, ATGA0521-20 µg, ATGA0521-50 µg, ATGA0521-100 µg, ATGA0521-250 µg, ATGA0521-500 µg, ATGA0521-1 mg, ATGA0521-10, ATGA0521-20, ATGA0521-50, ATGA0521-100, ATGA0521-250, ATGA0521-500, ATGA0521-1

References & Citations

Atshaves BP, et al: (2004) Mol Cell Biochem. 259 (1-2), 115-29; ; Nakamura T, et al: (2005) Diabestes Care. 28 (11), 2728-32

Other References

Ludvigsen M, et al. Proteomic Characterization of Colorectal Cancer Tissue from Patients Identifies Novel Putative Protein Biomarkers. (Curr Issues Mol Biol. 2021) {https://pubmed.ncbi.nlm.nih.gov/34563043/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cancer

NCBI Accession Number

NP_001434

Uniprot Accession Number

P07148

WB Description

The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP1/L-FABP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human FABP1/L-FABP protein; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Mouse Liver tissue lysate

IF Description

ICC/IF analysis of FABP1/L-FABP in Hep3B cells. The cell was stained with ATGA0521 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .
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