Human RAB5A antibody

Rab5a is a member of the RAS oncogene family. Rab5a is a small GTPase localized to the plasma membrane and early endosomes. It is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.

Product Specifications

Product Name Alternative

RAB5A member RAS oncogene family, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Rab5a (1-215aa) purified from E. coli.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

3A4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Rab5a, ATGA0519-10 µg, ATGA0519-20 µg, ATGA0519-50 µg, ATGA0519-100 µg, ATGA0519-250 µg, ATGA0519-500 µg, ATGA0519-1 mg, ATGA0519-10, ATGA0519-20, ATGA0519-50, ATGA0519-100, ATGA0519-250, ATGA0519-500, ATGA0519-1

References & Citations

Bucci C, et al., (1992) Cell 70 (5) : 715-28; ; Seachrist JL, et al., (2002) J. Biol. Chem. 277 (1) : 679-85

Other References

Gorji-Bahri G, et al. RAB5A is associated with genes involved in exosome secretion: Integration of bioinformatics analysis and experimental validation. (J Cell Biochem. 2021) {https://pubmed.ncbi.nlm.nih.gov/33225526/}; ; Gorji-Bahri G, et al. RAB5A effect on metastasis of hepatocellular carcinoma cell line via altering the pro-invasive content of exosomes. (Exp Mol Pathol. 2021) {https://pubmed.ncbi.nlm.nih.gov/33831402/}; ; Polla DL, et al. Biallelic variants in TMEM222 cause a new autosomal recessive neurodevelopmental disorder. (Genet Med. 2021) {https://pubmed.ncbi.nlm.nih.gov/33824500/}; ; Nanou A, et al. Endothelial Tpl2 regulates vascular barrier function via JNK-mediated degradation of claudin-5 promoting neuroinflammation or tumor metastasis. (Cell Rep. 2021) {https://pubmed.ncbi.nlm.nih.gov/34038728/}; ; Huang HW, Ryoo HD. Drosophila fabp is required for light-dependent Rhodopsin-1 clearance and photoreceptor survival. (PLoS Genet. 2021) {https://pubmed.ncbi.nlm.nih.gov/34714826/}; ; Patel M, et al. Spatial expression of IKK-alpha is associated with a differential mutational landscape and survival in primary colorectal cancer. (Br J Cancer. 2022) {https://pubmed.ncbi.nlm.nih.gov/35173303/}; ; Tang Y, et al. The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles. (Acta Pharm Sin B. 2022) {https://pubmed.ncbi.nlm.nih.gov/35847504/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_004153

Uniprot Accession Number

P20339

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rab5a antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Rab5a Transfected 293T cell lysate;

IF Description

ICC/IF analysis of Rab5a in U87MG cells. The cell was stained with ATGA0519 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .